2024-06-05 REL RCSB RCSB RCSB 2023-04-13 2024-02-21 2024-02-21 2024-06-05 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) HL128683 United States National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) HL160966 United States National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS) R21 AR077802 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI087846 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) R01AI152421 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35 GM139616 United States In situ human cardiac thick filament in the relaxed state Chen L Liu J Rastegarpouyani H Janssen PML Pinto JR Taylor KA myosin, cMyBP-C, titin, STRUCTURAL PROTEIN Chen L Liu J Rastegarpouyani H Janssen PML Pinto JR Taylor KA Structure of mavacamten-free human cardiac thick filaments within the sarcomere by cryoelectron tomography. Proc.Natl.Acad.Sci.USA US 121 e2311883121 e2311883121 2024 38386705 doi:10.1073/pnas.2311883121 1091-6490 0040 PNASA6 In situ human cardiac thick filament in the relaxed state In situ human cardiac thick filament in the relaxed state 0 Homo sapiens subtomogramAveraging tissue 6.8 ETHANE TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 6.0 6.0 GATAN K3 (6k x 4k) 1.5 1 16.5 FSC 0.143 CUT-OFF 757 5 11369 OTHER emd_40468.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 -96 -96 -96 192 192 192 813.46564 813.46564 813.46564 90.0 90.0 90.0 X Y Z -0.63768333 0.6925983 0.025764126 0.035650723 4.2368 4.2368 4.2368 0.115 AUTHOR Human cardiac thick filament in the relaxed state emd_40468_additional_9.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 813.46564 813.46564 813.46564 90.0 90.0 90.0 X Y Z -0.0 0.58710265 0.00013532865 0.005857759 4.2368 4.2368 4.2368 AUTHOR Eleven domains of C-zone titin-M emd_40468_additional_8.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 813.46564 813.46564 813.46564 90.0 90.0 90.0 X Y Z -0.0 0.53785026 0.00013953244 0.0056153317 4.2368 4.2368 4.2368 AUTHOR Eleven domains of C-zone titin-C emd_40468_additional_7.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 813.46564 813.46564 813.46564 90.0 90.0 90.0 X Y Z -0.0 0.49414912 0.00004613312 0.0031920148 4.2368 4.2368 4.2368 AUTHOR cMyBP-C domains C8, C9, C10 emd_40468_additional_6.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 196 196 499 -2 -2 -103 196 196 499 830.41284 830.41284 2114.1633 90.0 90.0 90.0 X Y Z -0.0 0.75944746 0.00010173139 0.0049774796 4.2368 4.2368 4.2368 AUTHOR Human cardiac myosin II tail of IHM-C segmented from three stacked repeating units of the thick filament backbone map emd_40468_additional_5.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 194 194 498 -1 -1 -1 194 194 498 821.9392 821.9392 2109.9265 90.0 90.0 90.0 X Y Z -0.0 0.47709548 0.000059927752 0.0029615113 4.2368 4.2368 4.2368 AUTHOR Human cardiac myosin II tail of IHM-S segmented from three stacked repeating units of the thick filament backbone map emd_40468_additional_4.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 194 194 498 -1 -1 -1 194 194 498 821.9392 821.9392 2109.9265 90.0 90.0 90.0 X Y Z -0.0 0.6948616 0.000090049485 0.004375262 4.2368 4.2368 4.2368 AUTHOR Human cardiac myosin II tail of IHM-D segmented from three stacked repeating units of the thick filament backbone map emd_40468_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 196 196 905 -2 -2 -2 196 196 905 830.41284 830.41284 3834.3042 90.0 90.0 90.0 X Y Z 0.0 0.7060923 0.00016153634 0.0060460353 4.2368 4.2368 4.2368 AUTHOR Curved layer composed of five human cardiac myosin II tail of IHM-S. emd_40468_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 196 196 905 -2 -2 -2 196 196 905 830.41284 830.41284 3834.3042 90.0 90.0 90.0 X Y Z 0.0 0.9313334 0.00024272998 0.008944272 4.2368 4.2368 4.2368 AUTHOR Curved layer composed of five human cardiac myosin II tail of IHM-D. emd_40468_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 198 198 906 -3 -3 -104 198 198 906 838.8864 838.8864 3838.541 90.0 90.0 90.0 X Y Z 0.0 0.94275284 0.00027452337 0.009601312 4.2368 4.2368 4.2368 AUTHOR Curved layer composed of five human cardiac myosin II tail of IHM-C. emd_40468_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 -96 -96 -96 192 192 192 813.46564 813.46564 813.46564 90.0 90.0 90.0 X Y Z -0.017828735 0.022321975 -0.0000031807187 0.0025794825 4.2368 4.2368 4.2368 AUTHOR Human cardiac thick filament in the relaxed state even half map emd_40468_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 -96 -96 -96 192 192 192 813.46564 813.46564 813.46564 90.0 90.0 90.0 X Y Z -0.017942645 0.021725532 -0.000008635585 0.0025703295 4.2368 4.2368 4.2368 AUTHOR Human cardiac thick filament in the relaxed state odd half map