2024-10-30 REL RCSB RCSB RCSB 2023-05-25 2024-05-29 2024-05-29 2024-10-30 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) HL153219 United States National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) NS112363 United States National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) NS111031 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM129547 United States Raw consensus map of TRPM2 chanzyme (E1114A) in the presence of Magnesium and ADP-ribose, open state Huang Y Kumar S Lu W Du J TRPM2 Chanzyme, Channel-enzyme, MEMBRANE PROTEIN Huang Y Kumar S Lee J Lu W Du J Coupling enzymatic activity and gating in an ancient TRPM chanzyme and its molecular evolution. Nat.Struct.Mol.Biol. US 31 1509 1521 2024 38773335 doi:10.1038/s41594-024-01316-4 1545-9985 EMD-40731 other EM volume
composite map
 EMD-40871 other EM volume
focused refinement map
 TRPM2 chanzyme (E1114A) incubated with Magnesium and ADP-ribose for 5s; ADP-ribose intact; open state TRPM2 chanzyme (E1114A) incubated with Magnesium and ADP-ribose for 5s; ADP-ribose intact; open state 0 1 Salpingoeca rosetta TRPM2 chanzyme (E1114A) incubated with Magnesium and ADP-ribose for 5s; ADP-ribose intact; open state. Salpingoeca rosetta Homo sapiens LEVO MQRARPGELVEVIMFRPTGKARVSNLDESMAMEFTDLRTRAMSSAAMIRQSVAAKTLLIENEDGKGSTRMEVQDFMKRFH MHASEDDKTGSPSTAWGTLRFPTKEATAPYLRLSVNDDPEDALLFVKAMLAQKYGETYDRPSLILSVTGGARNFTLPPRL ETAIAKGLRLAAQRTNAWVVTGGTNTGVMKLTGQIMEALSKTQSHFIPPTIGIATYGVIIGGDDMTRGEPPKIGLEYEMH KKDPPKTTPLDDNHNLFLLVDDGSTNKFGKEIKFRAAFENAAGQAFAAPVVTIVVQGGPGTLGTALQAVRQGTPIVVVDG SGLAADVLAYAYNFMHNPLTRFKSYTIDDLRQKVAQTFNPKSSQQLTNLLDSALECVQDPNLVVVYSLQESGIDEFDDCI LKAIFSSQGKLGNKLKQAMYFDQLDVAKRALSEASKNGQHNEIAACINDNLMAAMMHNKPHFVELYLGFDAKIYELKPSE EVAKTNITALDELPSFALAIEELYKREAKKPHSHVQRLVSLSNTDVLGRHYRVSTQRGDGTTRRIGRDLANTRAYNVLRM DQIFARLVSKDFSVNRDFTIYDSKYDKVPGIQFRRTAQASHMLFLWAICLDRFRMARHFWLIGDQSIINALVASRILERL STHRALQGPHLAEERAKMQHNAKKFEELAVGVLGECHGSDSHMASEMLHSKNDMFNKKNAINIAYDAKSLAFLSHPATQS VINADWYGHLKSVTSFWAVLFAFFFPFFVLPFINFSEDHAEQQVEAPRDFFTDAPRSSHSANSTTSGAHRLRRKFAKFYS APYTRFISDLLSHFVLCVVTSYFVLDKLEDTISAIEWILLVWFVALLLEELRQMIFCDGIAEYISDTWNRLDLIMITLFF VGFFTHASDPSNQDSKVVSKGIHAFLVVVLWLRFMRYYALSKNLGPKLIMMMEMMKDVSTFVFLLLIFLIGYGVAAQSLL SPDEDFSSRTFIGVLFRPYFQIYGELFLDDLNSEANCLGDTPFTECSRETVRMVPFFLAVYILGSNVLLVNLLIAMFNDT YMKVQEAAEDLWRKQNYELCAEYKDRPFLPAPFILLAHVHMLFMRLLRLCGVHTQEHEKIQDDETKRKITTFEALNTDKF LRRWERERQEMLEARVKMTNDNVVQAMGMMDQLLEHMISFRFSLDQQATKIKQEIRDDGLPSTEPTGLVSRTPSQPINRL NSAVAVHGHTAEAAEWYVPPEEYPKSGGVKRYLIDASMVPLSIMCPSYDPVEYTHPSVAAQPVWADPADPRKIKFNVKDE VNGKVVDRTSCHPSGISIDSNTGRPINPWGRTGMTGRGLLGKWGVNQAADTVVTRWKRSPDGSILERDGKKVLEFVAIQR QDNKMWAIPGGFVDNGEDVALTSGREFMEEALGMGTSADLMSAESKDSLAALFSSGTIVARIYCEDPRNTDNAWVETTCV NFHDESGRHAARLKLQGGDDAEHARWMMVHGGLNLFASHRTLLQHVTSALNAYF(APR)(MG)(MG)(MG)(MG)(MG) (CLR)(CLR)(CLR)(APR) singleParticle particle 8.5 8.0 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.9 1.9000000000000001 GATAN K3 BIOQUANTUM (6k x 4k) 0.02 49.0 1 3.87 FSC 0.143 CUT-OFF 8279 RANDOM ASSIGNMENT MAXIMUM LIKELIHOOD emd_40887.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 363.44 363.44 363.44 90.0 90.0 90.0 X Y Z -0.004055821 0.014831355 0.00014485385 0.0009846792 0.82600003 0.82600003 0.82600003 0.006 AUTHOR AB INITIO MODEL REAL emd_40887_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 363.44 363.44 363.44 90.0 90.0 90.0 X Y Z -0.010438155 0.02792339 0.00014393638 0.0009822766 0.82600003 0.82600003 0.82600003 AUTHOR emd_40887_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 363.44 363.44 363.44 90.0 90.0 90.0 X Y Z -0.023875158 0.030436147 0.00013981198 0.0024004118 0.82600003 0.82600003 0.82600003 AUTHOR emd_40887_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 440 440 440 0 0 0 440 440 440 363.44 363.44 363.44 90.0 90.0 90.0 X Y Z -0.028299848 0.03145101 0.00015168486 0.002409586 0.82600003 0.82600003 0.82600003 AUTHOR