Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (Map B)

2018-12-05 REL PDBe PDBe PDBe 2018-03-12 2016-08-24 2018-12-05 2018-12-05 Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (Map B) Llacer JL Hussain T Gordiyenko Y Ramakrishnan V Llacer JL Hussain T Saini AK Nanda JD Kaur S Gordiyenko Y Kumar R Hinnebusch AG Lorsch JR Ramakrishnan V Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition. Elife US 7 2018 30475211 doi:10.7554/eLife.39273 2050-084X EMD-4329 other EM volume EMD-4330 other EM volume EMD-4331 associated EM volume

Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)

Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model B) Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model B) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 Kluyveromyces lactis 1.8 singleParticle particle 0.15 6.5 20.0 MES 5.0 Magnessium acetate 80.0 Potassium acetate 10.0 Ammonium acetate 2.0 Dithiothreitol (DTT) 0.001 Zinc acetate 0.6 ATP 0.25 GDPCP Quantifoil R2/2 COPPER 400 CARBON CONTINUOUS GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK I

FEI POLARA 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.0 1.5 3.5 78000. 104478. NITROGEN 90.0 100.0 FEI FALCON III (4k x 4k) INTEGRATING 3 3600 1.1 40.0

Images were collected in movie-mode at 32 frames per second

FEI Falcon III

706713 Gctf 4V88 C1 FOURIER SPACE 3.5 FSC 0.143 CUT-OFF RELION 1.4 54699 NOT APPLICABLE RELION 1.4 ANGULAR RECONSTITUTION RELION 1.4 RELION 1.4 emd_4331.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 402.0 402.0 402.0 90.0 90.0 90.0 X Y Z -0.23156458 0.48808563 0.0000028441173 0.020816991 1.34 1.34 1.34 0.12 AUTHOR ::::EMDATABANK.org::::EMD-4331:::: For optimal visualization of all tRNA, eIF2 gamma and eIF3b and eIF3i beta propellers, gauss-filter the map by 1.2 and display it at 0.02 contour level. OTHER FSC RECIPROCAL 54. emd_4331_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 402.0 402.0 402.0 90.0 90.0 90.0 X Y Z -0.030826757 0.08852052 0.0000019152963 0.005577087 1.34 1.34 1.34 0.01 AUTHOR ::::EMDATABANK.org::::EMD-4331:::: emd_4331_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 402.0 402.0 402.0 90.0 90.0 90.0 X Y Z -0.03082226 0.0904532 -0.0000003622424 0.0055628694 1.34 1.34 1.34 0.01 AUTHOR ::::EMDATABANK.org::::EMD-4331::::