2024-11-13 REL RCSB RCSB RCSB 2024-04-23 2024-11-13 2024-11-13 2024-11-13 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM-136412 United States National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) AI170855 United States iSAT PNA - RA20-E-a1 Sheng K Dong X Williamson JR Ribosome, 50S intermediate, RNP Sheng K Dong X Aiyer S Lee J Marquardt S Lyumkis D Wiliamson JR Augmenting the Bacterial Ribosome Assembly Structurome using Anti-Sense Oligonucleotide Analogs To Be Published 0353 EMD-44483 other EM volume iSAT control - ctrl-C-a1 50S ribosomal subunit assembly intermediate iSAT control - ctrl-C-a1 50S ribosomal subunit assembly intermediate 0 Escherichia coli K-12 singleParticle particle 7.5 20.0 Tris-HCl 100.0 NH4Cl ammonium chloride 10.0 MgCl2 magnesium chloride 0.5 EDTA 6.0 beta-mercaptoethanol ETHANE 80
0.1 uM
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 1.0 3.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 BIOQUANTUM (6k x 4k) COUNTING 25.4 1 4.2 FSC 0.143 CUT-OFF
Best FSC was chosen in CryoSPARC
1898 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC
Non-Uniform refinement in CryoSPARC was used.
 cryoSPARC emd_44575.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 0 0 0 160 160 160 419.19998 419.19998 419.19998 90.0 90.0 90.0 X Y Z -0.48266777 1.3345102 0.0016750976 0.08071942 2.62 2.62 2.62 0.35 AUTHOR Aligned and rescaled to reference 4YBB 50S model emd_44575_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 419.99997 419.99997 419.99997 90.0 90.0 90.0 X Y Z -4.468433 5.3994904 0.0037050731 0.5215797 1.05 1.05 1.05 AUTHOR emd_44575_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 419.99997 419.99997 419.99997 90.0 90.0 90.0 X Y Z -4.608463 4.762511 0.0035249677 0.52927315 1.05 1.05 1.05 AUTHOR