2019-05-15 REL PDBe PDBe PDBe 2019-01-03 2019-05-15 2019-05-15 2019-05-15 Human post-catalytic spliceosome (P complex), focused refinement on Brr2 Fica SM Oubridge C Wilkinson ME Newman AJ Nagai KN Fica SM Oubridge C Wilkinson ME Newman AJ Nagai K A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation. Science US 363 710 714 2019 30705154 doi:10.1126/science.aaw5569 1095-9203 0038 SCIEAS EMD-4525 other EM volume EMD-4530 associated EM volume Human post-catalytic P complex spliceosome Human post-catalytic P complex spliceosome 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 Homo sapiens 3.0 singleParticle particle 1 7.9 20.0 HEPES 100.0 KCl 0.2 EDTA ETHANE 100 277 FEI VITROBOT MARK III
3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane..
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.5 3.5 130000. GATAN K2 SUMMIT (4k x 4k) COUNTING 60.0 1 C1 4.0 FSC 0.143 CUT-OFF RELION 3.0 82646 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 emd_4530.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 410 410 410 0 0 0 410 410 410 492.00003 492.00003 492.00003 90.0 90.0 90.0 X Y Z -0.039956793 0.2251631 -0.00002304385 0.0021851254 1.2 1.2 1.2 0.02 AUTHOR Human post-catalytic spliceosome (P complex), focused refinement on Brr2