2025-10-15 REL PDBe INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation citation_author em_admin _citation.country _citation.journal_abbrev _citation.journal_id_ASTM _citation.journal_id_CSD _citation.journal_id_ISSN _citation.pdbx_database_id_DOI _citation.pdbx_database_id_PubMed _citation.title _citation.year _em_admin.last_update DATABASE_REFERENCES citation citation_author em_admin _citation.journal_volume _citation.page_first _citation.page_last _citation_author.identifier_ORCID _em_admin.last_update PDBe PDBe 2024-09-16 2025-08-06 2025-08-06 2025-10-15 Max Planck Society Germany German Research Foundation (DFG) TRR 152/3, number 239283807 project P02 Germany Cryo-EM structure of mouse PMCA captured in E1-ATP in the presence of Calcium Vinayagam D Raunser S Sistel O Schulte U Constantin CE Prumbaum D Zolles G Fakler B Calcium pump, Ptype-Atpase, MEMBRANE PROTEIN Vinayagam D Sitsel O Schulte U Constantin CE Oosterheert W Prumbaum D Zolles G Fakler B Raunser S Molecular mechanism of ultrafast transport by plasma membrane Ca 2+ -ATPases. Nature UK 646 236 245 2025 40836084 doi:10.1038/s41586-025-09402-3 1476-4687 0006 NATUAS 9gsi FULLOVERLAP PDB 9GSD unspecified PDB 9GSE unspecified PDB 9GSF unspecified PDB 9GSG unspecified PDB 9GSH unspecified EMDB EMD-51549 associated EM volume
Cryo-EM structure of mouse PMCA captured in E1-ATP in the presence of Calcium
 mouse PMCA captured in E1-ATP in the presence of Calcium mouse PMCA captured in E1-ATP in the presence of Calcium 0 1
prepared as separate cDNAs for transient (co)transfection of tsA201 nptn/basi double KO cells
Mus musculus 0.18 Plasma membrane calcium-transporting ATPase 2 Mus musculus 0.134647141 1 Homo sapiens LEVO MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPGTAPDLEKRKQ IFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGESNEGCATAQGGAEDEGEAEAGWIEGAAILLS VICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQIKYGDLLPADGLFIQGNDLKIDESS LTGESDQVRKSVDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDKKAKQQDGAAAMEMQPLKSA EGGDADDKKKANMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLTECTPVYVQYFVKFFI IGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIP DPSSINAKTLELLVNAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLRQDYEPVRSQMPEEKLYKVYTFNSV RKSMSTVIKMPDESFRMYSKGASEIVLKKCCKILSGAGEARVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEP DWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRR IRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVA KEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLAL ATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLTLIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMM QLFNEINARKIHGERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIP TSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIH NFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSLEN LYFQGGDYKDDDDK Q9R0K7 7.2.2.10 PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER 0.000506196 1 ANP CALCIUM ION 4.0078e-05 1 CA MAGNESIUM ION 2.4305e-05 1 MG (2S)-1-{[(R)-hydroxy{[(1R,2R,3S,4R,5R,6S)-2,3,6-trihydroxy-4,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl icosa-5,8,11,14-tetraenoate 0.0010470879999999998 1 KXP singleParticle particle 2.0 7.4
Tris 20mM NaCl 150mM
 Quantifoil R1.2/1.3 COPPER CARBON CONTINUOUS GLOW DISCHARGE ETHANE 95 277 FEI VITROBOT MARK IV
The complex was monodisperse
 TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.1 2.5 55000.0 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 93.0 113.0 GIF Bioquantum 20 GATAN K3 (6k x 4k) 8076 3.5 57.0 1 CTFFIND 4.0 PHASE FLIPPING AND AMPLITUDE CORRECTION 6A69 1 C1 FOURIER SPACE 3.39 FSC 0.143 CUT-OFF cryoSPARC 4.0 non uniform refinement 300272 OTHER cryoSPARC 4.0
SGD Algorithm implemented in cryosparc
 MAXIMUM LIKELIHOOD cryoSPARC 4.0 3 RELION 3.1 emd_51549.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 273.0 273.0 273.0 90.0 90.0 90.0 X Y Z -3.6642325 5.107639 0.0026067954 0.08878863 0.91 0.91 0.91 0.33 AUTHOR 6A69 PDB experimental model OTHER REAL 161.400000000000006 emd_51549_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 273.0 273.0 273.0 90.0 90.0 90.0 X Y Z -0.6000306 1.1938697 0.0025668105 0.047761444 0.91 0.91 0.91 AUTHOR emd_51549_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 273.0 273.0 273.0 90.0 90.0 90.0 X Y Z -0.61062074 1.2799821 0.0025647325 0.046705898 0.91 0.91 0.91 AUTHOR