2025-05-07 REL PDBe PDBe PDBe 2024-11-25 2025-02-05 2025-02-05 2025-05-07 Medical Research Council (MRC, United Kingdom) MC_PC_17136 United Kingdom Other private SV40 large T antigen assembly with DNA in presence of ATP (3D variability component_001, frame_000). Shahid T De Biasio A AAA+ superfamily Helicase Substrate translocation DNA unwinding, DNA BINDING PROTEIN Shahid T Danazumi AU Tehseen M Alhudhali L Clark AR Savva CG Hamdan SM De Biasio A Structural dynamics of DNA unwinding by a replicative helicase. Nature UK 641 240 249 2025 40108462 doi:10.1038/s41586-025-08766-w 1476-4687 0006 NATUAS EMD-50289 consensus EM volume Large T antigen with DNA in presence of ATP. Large T antigen with DNA in presence of ATP. 0 1 2 3 Large T antigen 1 1 Betapolyomavirus macacae DNA 1 2 3 synthetic construct singleParticle particle 0.1 8.0
200 mM NaCl, 50 mM Tris-HCl (pH 8.0), 1 mM ATP.
 UltrAuFoil GOLD GRAPHENE OXIDE ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.0 2.5 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 9737 2.0 50.0 1 CTFFIND 4 RELION 4 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION 3.13 FSC 0.143 CUT-OFF cryoSPARC
Consensus reconstruction.
210910 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC 2 cryoSPARC emd_52182.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 320.63998 320.63998 320.63998 90.0 90.0 90.0 X Y Z -1.6045976 14.96546 0.03373507 0.44445416 0.835 0.835 0.835 1.05 AUTHOR 3D variability volume post-processed by EMReady2 (component_001_frame_000). emd_52182_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 320.63998 320.63998 320.63998 90.0 90.0 90.0 X Y Z -0.9040548 0.83149934 0.00089192606 0.102614336 0.835 0.835 0.835 AUTHOR Unfiltered and unmasked gold-standard half-map (consensus refinement). emd_52182_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 384 384 384 0 0 0 384 384 384 320.63998 320.63998 320.63998 90.0 90.0 90.0 X Y Z -0.85015196 0.8855882 0.0008931375 0.10283253 0.835 0.835 0.835 AUTHOR Unfiltered and unmasked gold-standard half-map (consensus refinement).