<emd emdb_id="EMD-5331" version="3.0.1.1" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_1_1/emdb_relaxed.xsd">
    <admin>
        <current_status>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2011-08-15</deposition>
            <header_release>2011-08-29</header_release>
            <map_release>2011-12-06</map_release>
            <update>2012-03-19</update>
        </key_dates>
        <title>The cryoEM structure of the bacteriophage phiKZ polysheath</title>
        <authors_list>
            <author>Aksyuk AA</author>
            <author>Kurochkina LP</author>
            <author>Fokine A</author>
            <author>Mesyanzhinov VV</author>
            <author>Rossmann MG</author>
        </authors_list>
        <keywords>homopolymer of the bacteriophage tail sheath protein</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Aksyuk AA</author>
                    <author order="2">Kurochkina LP</author>
                    <author order="3">Fokine A</author>
                    <author order="4">Forouhar F</author>
                    <author order="5">Mesyanzhinov VV</author>
                    <author order="6">Tong L</author>
                    <author order="7">Rossmann MG</author>
                    <title>Structural conservation of the myoviridae phage tail sheath protein fold.</title>
                    <journal>STRUCTURE</journal>
                    <volume>19</volume>
                    <first_page>1885</first_page>
                    <last_page>1894</last_page>
                    <year>2011</year>
                    <external_references type="PUBMED">22153511</external_references>
                    <external_references type="DOI">doi:10.1016/j.str.2011.09.012</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>3j0i</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Bacteriophage phiKZ polysheath</name>
        <supramolecule_list>
            <sample_supramolecule supramolecule_id="1000">
                <name>Bacteriophage phiKZ polysheath</name>
                <number_unique_components>1</number_unique_components>
            </sample_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name synonym="polysheaths">bacteriophage phiKZ polysheaths</name>
                <natural_source database="NCBI">
                    <organism ncbi="169683">Pseudomonas phage phiKZ</organism>
                </natural_source>
                <recombinant_exp_flag>true</recombinant_exp_flag>
                <recombinant_expression database="NCBI" />
                <sequence />
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>OTHER</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI/PHILIPS CM200FEG</microscope>
                    <illumination_mode>SPOT SCAN</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <specimen_holder_model>SIDE ENTRY, EUCENTRIC</specimen_holder_model>
                    <specimen_holder>Eucentric</specimen_holder>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <final_reconstruction>
                    <resolution res_type="BY AUTHOR" units="&#8491;">19.0</resolution>
                    <resolution_method>FSC 0.5 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>SPIDER</name>
                        </software>
                    </software_list>
                </final_reconstruction>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="54001">
        <file>emd_5331.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>240</col>
            <row>240</row>
            <sec>240</sec>
        </dimensions>
        <origin>
            <col>-120</col>
            <row>-120</row>
            <sec>-120</sec>
        </origin>
        <spacing>
            <x>240</x>
            <y>240</y>
            <z>240</z>
        </spacing>
        <cell>
            <a units="&#8491;">597.6</a>
            <b units="&#8491;">597.6</b>
            <c units="&#8491;">597.6</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-5.54504299</minimum>
            <maximum>8.81647396</maximum>
            <average>-0.00043717</average>
            <std>0.83758986</std>
        </statistics>
        <pixel_spacing>
            <x units="&#8491;">2.49</x>
            <y units="&#8491;">2.49</y>
            <z units="&#8491;">2.49</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>2.6</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <annotation_details>This is a map of the bacteriophage phiKZ polysheath</annotation_details>
        <details>::::EMDATABANK.org::::EMD-5331::::</details>
    </map>
    <interpretation>
        <figure_list>
            <figure>
                <file>emd_5331.jpg</file>
            </figure>
        </figure_list>
    </interpretation>
</emd>