2024-03-27 REL PDBj PDBj PDBj 2017-10-02 2017-04-19 2018-02-21 2024-03-27 human ferritin mutant - E-helix deletion Lee SG Yoon HR ferritin, cage, drug delivery, TRANSPORT PROTEIN Ahn B Lee SG Yoon HR Lee JM Oh HJ Kim HM Jung Y Four-fold Channel-Nicked Human Ferritin Nanocages for Active Drug Loading and pH-Responsive Drug Release Angew. Chem. Int. Ed. Engl. GE 57 2909 2913 2018 29359486 doi:10.1002/anie.201800516 1521-3773 0179 ACIEAY EMD-6714 other EM volume 5yi5 FULLOVERLAP human ferritin E-helix deletion mutant human ferritin E-helix deletion mutant 0 1 Homo sapiens 0.42 Ferritin heavy chain Homo sapiens 0.020608104999999998 24 Escherichia coli LEVO MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGR IFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMG APESGLAEYLFDKHTLG P02794 1.16.3.1 singleParticle particle 0.05 7.2 150.0 NaCl sodium chloride 50.0 Tris tris(hydroxymethyl)aminomethane
buffers were titrated with HCl to pH7.2
 Quantifoil R1.2/1.3 COPPER 400 CARBON HOLEY GRAPHENE OXIDE CONTINUOUS GLOW DISCHARGE AIR 0.039
the grid was coated with graphene oxide prior to use
 ETHANE 100 276 FEI VITROBOT MARK IV
blot for 9 seconds before plunging.
this sample was monodisperse
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.002 47000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON II (4k x 4k) 2-29 1.8 2.0 1 623510 4Y08
human ferritin
 3.0 FSC 0.143 CUT-OFF RELION 2.0
relion2.0 program was used for the reconstruction
429135 ANGULAR RECONSTITUTION RELION 2.0 ANGULAR RECONSTITUTION RELION 2.0 RELION 2.0 emd_6830.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 178.8544 178.8544 178.8544 90.0 90.0 90.0 X Y Z -0.45643964 0.88335246 0.021175088 0.08361874 1.3973 1.3973 1.3973 0.3 AUTHOR human ferritin heavy chain complexed with doxorubicin 4Y08 A 5-160 PDB experimental model FLEXIBLE FIT REAL 29.559999999999999