2025-12-17 REL RCSB RCSB RCSB 2025-06-09 2025-08-27 2025-08-27 2025-12-17 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) R35 HL135823 United States National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) R35 HL171334 United States National Institutes of Health/Office of the Director S10OD030275 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R24 GM145965 United States American Heart Association N028347 United States Nanodisc-embedded human TF/FVIIa/XK1 in complex with 10H10 Fab Photenhauer AL Sedzro JC Morrissey JH Ohi MD complex, nanodisc, blood clotting Sedzro JC Photenhauer AL Birkle F Meze K Mortenson A Duckworth C Wen PC Kearns S Cianfrocco MA Tajkhorshid E Ohi MD Morrissey JH Cryo-EM structure of the tissue factor/factor VIIa complex with a factor X mimetic reveals a novel allosteric mechanism. Blood US 146 2833 2842 2025 40811856 doi:10.1182/blood.2025029430 1528-0020 EMD-71090 other EM volume EMDB EMD-71090 other EM volume EMDB EMD-71093 associated EM volume Complex of factor VIIa, XK1 and the Fab fragment of antibody 10H10, all bound to tissue factor embedded in a nanodisc membrane bilayer. Complex of factor VIIa, XK1 and the Fab fragment of antibody 10H10, all bound to tissue factor embedded in a nanodisc membrane bilayer. 0 1 2 3 4 5 6 7
Map with nanodisc.
 Human 10H10 antibody Fab 1 6 7 Mus musculus XK1 chimeric protein 1 3 4 Homo sapiens Human activated factor VIIa 1 1 2 Homo sapiens Human MBP-tagged tissue factor 1 5 Homo sapiens Human factor VIIa (FVIIa) light chain Homo sapiens Homo sapiens LEVO ANAFL(CGU)(CGU)LRPGSL(CGU)R(CGU)CK(CGU)(CGU)QCSF(CGU)(CGU)AR(CGU)IFKDA(CGU)RTKLF Human factor VIIa (FVIIa) protease domain Homo sapiens Homo sapiens LEVO IVGGKVCPKGECPWQVLLLVNGAQLCGGTLINTIWVVSAAHCFDKIKNWRNLIAVLGEHDLSEHDGDEQSRRVAQVIIPS Human factor X (FX) light chain Homo sapiens Homo sapiens LEVO ANSFL(CGU)(CGU)MKKGHL(CGU)R(CGU)CM(CGU)(CGU)TCSY(CGU)(CGU)AR(CGU)VF(CGU)DSDKTN Human TFPI Kunitz domain 1 (K1) Homo sapiens Homo sapiens LEVO TDTELPPLKLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRD Human tissue factor (TF) Homo sapiens Escherichia coli BL21(DE3) LEVO TVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVES Human 10H10 antibody Fab heavy chain Mus musculus LEVO QVHLQQSGAELMKPGASVKISCKASGYTFITYWIEWVKQRPGHGLEWIGDILPGSGSTNYNENFKGKATFTADSSSNTAY Human 10H10 antibody Fab light chain Mus musculus LEVO DIVMTQSPSSLTVTAGEKVTMSCKSSQSLLSSGNQKNYLTWYQQIPGQPPKLLIYWASTRESGVPDRFTGSGSGTDFTLT singleParticle particle 7.4 Quantifoil R2/2 COPPER 200 CARBON GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 105000.0 20 GATAN K3 (6k x 4k) 1 7132 50.0 1 3440922 NONE 3.2 FSC 0.143 CUT-OFF cryoSPARC 4.5.3 Non-uniform refinement 91001 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD emd_71093.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 420 420 420 0 0 0 420 420 420 350.28 350.28 350.28 90.0 90.0 90.0 X Y Z -0.3066422 0.61088324 -0.0000669639 0.010212363 0.834 0.834 0.834 0.04 AUTHOR Unsharpened cryoem map of TF/FVIIa/XK1 complex in nanodiscs without particle subtraction 9P0X PDB experimental model
Model generated from associated entry
 emd_71093_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 420 420 420 0 0 0 420 420 420 350.28 350.28 350.28 90.0 90.0 90.0 X Y Z -0.4490036 0.763646 0.00014557126 0.043609627 0.834 0.834 0.834 AUTHOR half map A emd_71093_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 420 420 420 0 0 0 420 420 420 350.28 350.28 350.28 90.0 90.0 90.0 X Y Z -0.4759427 0.77710265 0.0001524169 0.04416872 0.834 0.834 0.834 AUTHOR half map B