2025-12-17 REL RCSB INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY INITIAL_RELEASE REPOSITORY DATABASE_REFERENCES citation em_admin _citation.journal_volume _citation.page_first _em_admin.last_update EXPERIMENTAL_SUMMARY citation em_admin _citation.journal_volume _citation.page_first _em_admin.last_update RCSB RCSB 2025-06-09 2025-10-22 2025-10-22 2025-12-17 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM134867 United States Cas1-Cas2/3 integrase bound to foreign dsDNA fragment Henriques WS Bowman J Hall LN Gauvin CG Wei H Kuang H Zimanyi CM Eng ET Santiago-Frangos A Wiedenheft B CRISPR, integrase, type I-F, RECOMBINATION Henriques WS Bowman J Hall LN Gauvin CC Wei H Kuang H Zimanyi CM Eng ET Santiago-Frangos A Wiedenheft B Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci. Structure UK 33 2058 2025 41072406 doi:10.1016/j.str.2025.09.007 0969-2126 2005 STRUE6 9p1d FULLOVERLAP PDB 9P11 unspecified
9P11 contains the same protein complex in an apo state
 EMDB EMD-71097 associated EM volume
Cas1-Cas2/3 integrase bound to foreign dsDNA fragment
 Cas1-2/3 with bound foreign dsDNA Cas1-2/3 with bound foreign dsDNA 0 1 2 3 4 0.415 Cas1-2/3 1 1 2 Pseudomonas aeruginosa dsDNA 1 3 4 Pseudomonas aeruginosa CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST Pseudomonas aeruginosa 0.12127367999999998 2 Escherichia coli LEVO MNILLVSQCEKRALSETRRILDQFAERRGERTWQTPITQAGLDTLRRLLKKSARRNTAVACHWIRGRDHSELLWIVGDAS RFNAQGAVPTNRTCRDILRKEDENDWHSAEDIRLLTVMAALFHDIGKASQAFQAKLRNRGKPMADAYRHEWVSLRLFEAF VGPGSSDEDWLRRLADKRETGDAWLSQLARDDRQSAPPGPFQKSRLPPLAQAVGWLIVSHHRLPNGDHRGSASLARLPAP IQSQWCGARDADAKEKAACWQFPHGLPFASAHWRARTALCAQSMLERPGLLARGPALLHDSYVMHVSRLILMLADHHYSS LPADSRLGDPNFPLHANTDRDSGKLKQRLDEHLLGVALHSRKLAGTLPRLERQLPRLARHKGFTRRVEQPRFRWQDKAYD CAMACREQAMEHGFFGLNLASTGCGKTLANGRILYALADPQRGARFSIALGLRSLTLQTGQAYRERLGLGDDDLAILVGG SAARELFEKQQERLERSGSESAQELLAENSHVHFAGTLEDGPLREWLGRNSAGNRLLQAPILACTIDHLMPASESLRGGH QIAPLLRLMTSDLVLDEVDDFDIDDLPALSRLVHWAGLFGSRVLLSSATLPPALVQGLFEAYRSGREIFQRHRGAPGRAT EIRCAWFDEFSSQSSAHGAVTSFSEAHATFVAQRLAKLEQLPPRRQAQLCTVHAAGEARPALCRELAGQMNTWMADLHRC HHTEHQGRRISFGLLRLANIEPLIELAQAILAQGAPEGLHVHLCVYHSRHPLLVRSAIERQLDELLKRSDDDAAALFARP TLAKALQASTERDHLFVVLASPVAEVGRDHDYDWAIVEPSSMRSIIQLAGRIRRHRSGFSGEANLYLLSRNIRSLEGQNP AFQRPGFETPDFPLDSHDLHDLLDPALLARIDASPRIVEPFPLFPRSRLVDLEHRRLRALMLADDPPSSLLGVPLWWQTP ASLSGALQTSQPFRAGAKERCYALLPDEDDEERLHFSRYEEGTWSNQDNLLRNLDLTYGPRIQTWGTVNYREELVAMAGR EDLDLRQCAMRYGEVRLRENTQGWSYHPYLGFKKYN Q02ML8 3.1.-.- CRISPR-associated endonuclease Cas1 Pseudomonas aeruginosa 0.036154844
Strep-tagged Cas1
4 Escherichia coli LEVO MDDISPSELKTILHSKRANLYYLQHCRVLVNGGRVEYVTDEGRHSHYWNIPIANTTSLLLGTGTSITQAAMRELARAGVL VGFCGGGGTPLFSANEVDVEVSWLTPQSEYRPTEYLQRWVGFWFDEEKRLVAARHFQRARLERIRHSWLEDRVLRDAGFA VDATALAVAVEDSARALEQAPNHEHLLTEEARLSKRLFKLAAQATRYGEFVRAKRGSGGDPANRFLDHGNYLAYGLAATA TWVLGIPHGLAVLHGKTRRGGLVFDVADLIKDSLILPQAFLSAMRGDEEQDFRQACLDNLSRAQALDFMIDTLKDVAQRS TVSA Q02ML7 3.1.-.- DNA Strand 1 Pseudomonas aeruginosa 0.010377701999999999 1 (DA)(DA)(DA)(DA)(DA)(DC)(DC)(DT)(DG)(DG)(DA)(DC)(DT)(DA)(DC)(DT)(DA)(DC)(DA)(DA) (DC)(DC)(DT)(DT)(DC)(DG)(DC)(DT)(DT)(DT)(DT)(DT)(DG)(DG) DNA DNA Strand 2 Pseudomonas aeruginosa 0.009959434999999999 1 (DT)(DT)(DT)(DT)(DT)(DG)(DC)(DG)(DA)(DA)(DG)(DG)(DT)(DT)(DG)(DT)(DA)(DG)(DT)(DA) (DG)(DT)(DC)(DC)(DA)(DG)(DG)(DA)(DA)(DA)(DA)(DA) DNA singleParticle particle 1.7 7.5 Quantifoil R1.2/1.3 COPPER 300 GLOW DISCHARGE ETHANE 100 277 FEI VITROBOT MARK IV
Monodisperse peak on SEC
 TFS KRIOS OTHER OTHER FIELD EMISSION GUN 300 0.9 2.8000000000000003 96000.0 FEI FALCON IV (4k x 4k) 5552 6.0 51.78 1 1083100
crYolo
 cryoSPARC PHASE FLIPPING AND AMPLITUDE CORRECTION C1 3.31 FSC 0.143 CUT-OFF cryoSPARC 28665 COMMON LINE COMMON LINE emd_71097.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 333.2 333.2 333.2 90.0 90.0 90.0 X Y Z -0.55606955 1.1100007 0.0002923546 0.030371787 0.833 0.833 0.833 0.178 AUTHOR AlphaFold in silico model 8flj PDB experimental model RIGID BODY FIT emd_71097_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 333.2 333.2 333.2 90.0 90.0 90.0 X Y Z -0.90047634 1.6217088 0.00083212985 0.08791426 0.833 0.833 0.833 AUTHOR emd_71097_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 400 400 400 0 0 0 400 400 400 333.2 333.2 333.2 90.0 90.0 90.0 X Y Z -0.8946036 1.5246046 0.0008605786 0.08882834 0.833 0.833 0.833 AUTHOR