2024-03-13 REL RCSB RCSB RCSB 2018-03-27 2018-04-04 2019-07-24 2024-03-13 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) AI011219 United States CryoEM structure of human enterovirus D68 emptied particle (after incubation with low molecular weight heparin) Liu Y Rossmann MG virus, genome release, receptor Baggen J Liu Y Lyoo H van Vliet ALW Wahedi M de Bruin JW Roberts RW Overduin P Meijer A Rossmann MG Thibaut HJ van Kuppeveld FJM Bypassing pan-enterovirus host factor PLA2G16. Nat Commun UK 10 3171 3171 2019 31320648 doi:10.1038/s41467-019-11256-z 2041-1723 EMD-7632 other EM volume EMD-7633 other EM volume EMD-7634 other EM volume EMD-7636 other EM volume 6cv4 FULLOVERLAP Enterovirus D68 Enterovirus D68 0 1 2 3 Enterovirus D68 947 Homo sapiens VIRION STRAIN false true viral protein 1 Enterovirus D68 947 0.032957449 1 LEVO IESIIKTATDTVKSEINAELGVVPSLNAVETGATSNTEPEEAIQTRTVINQHGVSETLVENFLSRAALVSKRSFEYKDHT SSAAQTDKNFFKWTINTRSFVQLRRKLELFTYLRFDAEITILTTVAVNGSSNNTYMGLPNLTLQAMFVPTGALTPEKQDS FHWQSGSNASVFFKISDPPARMTIPFMCINSAYSVFYDGFAGFEKSGLYGINPADTIGNLCVRIVNEHQPVGFTVTVRVY MKPKHIKAWAPRPPRTLPYMSIANANYKGKKRAPNALNAIIGNRDSVKTMPHNIVTT A0A0X7Z9B1 viral protein 3 Enterovirus D68 947 0.027170895 1 LEVO GVPTYLLPGSGQFLTTDDHSSAPVLPCFNPTPEMHIPGQVRNMLEVVQVESMMEINNTESAVGMERLKVDISALTDVDQL LFNIPLDIQLDGPLRNTLVGNISRYYTHWSGSLEMTFMFCGSFMATGKLILCYTPPGGSCPTTRETAMLGTHIVWDFGLQ SSVTLIIPWISGSHYRMFNNDAKSTNANVGYVTCFMQTNLIVPSESSDTCSLIGFIAAKDDFSLRLMRDSPDIGQIDHLH AAEAAYQ E9RIT6 viral protein 2 Enterovirus D68 947 0.027595215 1 LEVO SPSAEACGYSDRVLQLKLGNSAIVTQEAANYCCAYGEWPNYLPDHEAVAIDKPTQPETATDRFYTLKSVKWEAGSTGWWW KLPDALNNIGMFGQNVQHHYLYRSGFLIHVQCNATRFHQGALLVVAIPEHQRGAHNTNTSPGFDDIMKGEEGGTFNHPYV LDDGTSLACATIFPHQWINLRTNNSATIVLPWMNAAPMDFPLRHNQWTLAIIPVVPLGTRTMSSMVPITVSIAPMCCEFN GLRHAITQ A0A097ZN88 singleParticle particle 7.2
phosphate buffer
 Homemade COPPER 400 CARBON CONTINUOUS GLOW DISCHARGE 0.02 ETHANE 85 298 GATAN CRYOPLUNGE 3 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 0.6 4.5 22500.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 3838 3710 2-35 1 872 7.0 33.0 1 18049
randomly assign orientations to particles
 I FOURIER SPACE 3.03 FSC 0.143 CUT-OFF jspr 9226 RANDOM ASSIGNMENT jspr PROJECTION MATCHING 0.5 jspr RELION 2.0.5 emd_7635.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 16 16 16 352 352 352 457.59998 457.59998 457.59998 90.0 90.0 90.0 X Y Z -237.044340000000005 297.381160000000023 0.15679918 16.330873 1.3 1.3 1.3 41.0 AUTHOR sharpened map in which the inner density is masked out RIGID BODY FIT correlation coefficient REAL emd_7635_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 16 16 16 352 352 352 457.59998 457.59998 457.59998 90.0 90.0 90.0 X Y Z -22.232486999999999 32.317604000000003 0.0752628 2.1571743 1.3 1.3 1.3 AUTHOR unsharpened half map in which the inner density is retained emd_7635_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 16 16 16 352 352 352 457.59998 457.59998 457.59998 90.0 90.0 90.0 X Y Z -17.109337 34.706040000000002 0.08097123 2.1796422 1.3 1.3 1.3 AUTHOR unsharpened half map in which the inner density is retained